Stretchable Microelectrode Arrays with Microneedles for Reliable Electrophysiological Recording of Human Heart and Brain Organoids
AI Summary
Stretchable 3D microelectrode array with microneedles fabricated via scalable wafer-level stud-bump bonding enables minimally destructive, stable, long-term electrophysiological interfacing with organoids.
3D microneedle electrodes yield higher signal-to-noise ratio and greater recording stability than 2D or planar 3D electrodes in heart and brain organoids.
Compatible with quantitative pharmacological profiling, the platform supports precise drug screening and scalable disease modelling due to manufacturable, tissue-compliant interfaces.
Adv Sci (Weinh). 2026 May 20:e75778. doi: 10.1002/advs.75778. Online ahead of print.
ABSTRACT
Real-time, non-destructive monitoring of electrophysiological dynamics of 3D organoids is imperative for advancing disease modeling and high-throughput drug screening. However, obtaining continuous, reliable signals remains difficult due to the destructive nature of penetrating probes and the unreliable contact issue prone to surface recordings. Here, we present a stretchable 3D microelectrode array with microneedles (3D MN-sMEA) fabricated via a scalable wafer-level stud-bump bonding process for minimally destructive and stable monitoring. We achieve high-fidelity, reliable electrophysiological recordings of both human iPSC-derived heart and cerebral models. Compared with 2D and 3D planar microelectrodes, 3D microelectrodes with microneedles achieve a higher signal-to-noise ratio and greater long-term recording stability. Furthermore, quantitative pharmacological profiling validates its ability to enable precise drug screening. By combining scalable manufacturing with flexible, tissue-compliant interfaces, our approach enables stable, minimally invasive, and long-term electrophysiological monitoring of 3D organoids for scalable disease modeling and drug discovery.
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