Evidence
![](https://psychiatryai.com/wp-content/uploads/2023/04/psychiatryai_com.webp)
J Cell Sci. 2024 May 3:jcs.261961. doi: 10.1242/jcs.261961. Online ahead of print.
ABSTRACT
Hepatocyte organoids (HOs) generated in vitro recently are powerful tools for liver regeneration. However, the reported HOs were mostly fetal in nature with low expression levels of metabolic genes characteristic of adult liver functions, hampering their application in studying metabolic regulations and therapeutic testing for liver disorders. We report development of novel culture conditions that contains optimized levels of Triiodothyronine (T3) with the removal of growth factors, enabled successful generation of mature hepatocyte organoids (MHOs) with metabolic functions characteristic of adult livers of both mouse and human origins. We showed that the MHOs can be used to study various metabolic functions including bile and urea production, zonal metabolic gene expression, and metabolic alterations in both alcoholic and non-alcoholic fatty liver diseases as well hepatocyte proliferation, injury, and cell fate changes. Notably, the MHOs derived from human fetal hepatoblasts also showed improved hepatitis B virus (HBV) infection. Therefore, these MHOs provide a powerful in vitro model for studies of human liver physiology and diseases. The human MHOs are potentially a robust research tools for therapeutic development as well.
PMID:38700490 | DOI:10.1242/jcs.261961
![Google](https://www.google.com/images/branding/googlelogo/2x/googlelogo_light_color_92x30dp.png)
![Google Keep](https://www.gstatic.com/images/branding/product/1x/keep_48dp.png)
![Share on Linkedin](https://psychiatryai.com/wp-content/uploads/2023/10/linkedin-logo-png-2048-1.png)
Estimated reading time: 4 minute(s)
Latest: Psychiatryai.com #RAISR4D Evidence
![](/wp-content/uploads/2024/04/bd462cc11bcf0bd0d0d6f1d0f8b7cd04-modified-1.png)
Cool Evidence: Engaging Young People and Students in Real-World Evidence
![](/wp-content/uploads/2024/04/bd462cc11bcf0bd0d0d6f1d0f8b7cd04-modified-1.png)
Real-Time Evidence Search [Psychiatry]
![](/wp-content/uploads/2024/04/pubmed.png)
AI Research
![](/wp-content/uploads/2024/05/Il5nR_nf_400x400-modified-1.png)
Generation and characterization of mature hepatocyte organoids for liver metabolic studies
![](https://psychiatryai.com/wp-content/uploads/2023/04/psychiatryai_com.webp)
🌐 90 Days
AI Virtual Reality Related Evidence Matrix
Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids Regulatory pathways and therapeutic potential of PDE4 in liver pathophysiology Hepatic extracellular ATP/adenosine dynamics in zebrafish models of alcoholic and metabolic steatotic liver disease A novel experimental model of MetALD in male mice recapitulates key features of severe alcohol-associated hepatitis Inflammatory liver diseases and susceptibility to sepsis TET3 boosts hepatocyte autophagy and impairs non-alcoholic fatty liver disease by increasing ENPP1 promoter hypomethylation Galectin-3 and Severity of Liver Fibrosis in Metabolic Dysfunction-Associated Fatty Liver Disease Patients with Celiac Disease Have High Prevalence of Fatty Liver and Metabolic Syndrome Amelioration of NAFLD by sleeve gastrectomy-triggered hepatocyte regeneration in mice - experimental research Role of lactate and lactate metabolism in liver diseases (Review) Liver-specific mitochondrial amidoxime-reducing component 1 (Mtarc1) knockdown protects the liver from diet-induced MASH in multiple mouse models Generation of human cerebral organoids with a structured outer subventricular zone Spatial genomics: mapping human steatotic liver disease New nomenclature for fatty liver disease Design optimization of geometrically confined cardiac organoids enabled by machine learning techniques The Transition in Terminology: From Non-alcoholic Fatty Liver Disease (NAFLD) to Metabolic Dysfunction-associated Fatty Liver Disease (MAFLD) to Metabolic-associated Steatotic Liver Disease (MASLD) Advances in management of metabolic dysfunction-associated steatotic liver disease: from mechanisms to therapeutics Pivotal role of intestinal cholesterol and nuclear receptor LXR in metabolic liver steatohepatitis and hepatocarcinoma Kupffer cell diversity maintains liver function in alcohol-associated liver disease An unbiased ranking of murine dietary models based on their proximity to human metabolic dysfunction-associated steatotic liver disease (MASLD) Coexistence of metabolic-associated fatty liver disease and autoimmune or toxic liver disease The deleterious effects and potential therapeutic strategy of fluorene-9-bisphenol on circadian activity and liver diseases in zebrafish and mice Cyanotoxin cylindrospermopsin disrupts lipid homeostasis and metabolism in a 3D in vitro model of the human liver FGL1 and FGL2: emerging regulators of liver health and disease Pediatric metabolic (dysfunction)-associated fatty liver disease: current insights and future perspectives Hericium erinaceus polysaccharides ameliorate nonalcoholic fatty liver disease via gut microbiota and tryptophan metabolism regulation in an aged laying hen model Spinal cord injury-induced metabolic impairment and steatohepatitis develops in non-obese rats and is exacerbated by premorbid obesity PSTPIP2 protects against alcoholic liver injury and invokes STAT3-mediated suppression of apoptosis Novel IL-4/HB-EGF-dependent crosstalk between eosinophils and macrophages controls liver regeneration after ischaemia and reperfusion injury Design and synthesis of novel JNK inhibitors targeting liver pyruvate kinase for the treatment of non-alcoholic fatty liver disease and hepatocellular carcinoma Impact of the diagnosis of metabolic dysfunction-associated fatty liver disease and non-alcoholic fatty liver disease in patients undergoing liver transplantation for hepatocellular carcinoma MAFLD as part of systemic metabolic dysregulation MAFLD as part of systemic metabolic dysregulation Interplay between metabolic dysfunction-associated fatty liver disease and renal function: An intriguing pediatric perspective Resmetirom, the long-awaited first treatment for metabolic dysfunction-associated steatohepatitis and liver fibrosis? RNA modifications in the progression of liver diseases: from fatty liver to cancer RNA modifications in the progression of liver diseases: from fatty liver to cancer Gut-liver axis: Potential mechanisms of action of food-derived extracellular vesicles Liver histology is associated with long-term clinical outcomes in patients with metabolic dysfunction-associated steatohepatitis Kaempferol efficacy in metabolic diseases: Molecular mechanisms of action in diabetes mellitus, obesity, non-alcoholic fatty liver disease, steatohepatitis, and atherosclerosis Cardiometabolic and Metabolic Profiles of Lean/Normal, Overweight and Obese Patients with Nonalcoholic Fatty Liver Disease The fatty acid omega hydroxylase genes (CYP4 family) in the progression of metabolic dysfunction-associated steatotic liver disease (MASLD): An RNA sequence database analysis and review Single-Cell Data Analysis Reveals Critical Hepatic Cells Subpopulations in the Progression of Non-alcoholic Fatty Liver Disease to Non-Alcoholic Steatohepatitis Monitoring disease progression in metabolic dysfunction-associated steatotic liver disease Monitoring disease progression in metabolic dysfunction-associated steatotic liver disease Targeting metabolic-associated fatty liver disease in diabetic kidney disease: A call to action Guidelines for the prevention and treatment of metabolic dysfunction-associated (non-alcoholic) fatty liver disease (Version 2024) Guidelines for the prevention and treatment of metabolic dysfunction-associated (non-alcoholic) fatty liver disease (Version 2024) Multiscale 3D genome organization underlies duck fatty liver with no adipose inflammation or serious injury 3D microscaffolds with triple-marker sensitive nanoprobes for studying fatty liver disease in vitro Associations of dietary sugars with liver stiffness in Latino adolescents with obesity differ on PNPLA3 and liver disease severity AMPED study: Protocol for a randomized controlled trial of different doses of aerobic exercise training Metabolic dysfunction-associated fatty liver disease and low muscle strength: A comment Does the liver facilitate aging-related cognitive impairment: Conversation between liver and brain during exercise? Comparative study of MAFLD as a predictor of metabolic disease treatment for NAFLD Analysis of non-alcoholic fatty liver disease differences from metabolic dysfunction-associated fatty liver disease based on clinical features Glycyrrhizin and the Related Preparations: An Inspiring Resource for the Treatment of Liver Diseases Mitochondrial ACSS1-K635 acetylation knock-in mice exhibit altered metabolism, cell senescence, and nonalcoholic fatty liver disease Integrated transcriptomics and histopathology approach identifies a subset of rejected donor livers with potential suitability for transplantation Incretin-based investigational therapies for the treatment of MASLD/MASH Metabolic Dysfunction-associated Fatty Liver Disease: An Urgent Call for Global Action Increased hepatoprotective effects of the novel farnesoid X receptor agonist INT-787 versus obeticholic acid in a mouse model of nonalcoholic steatohepatitis Paeonol attenuates nonalcoholic steatohepatitis by regulating intestinal flora and AhR/NLRP3/Caspase-1 metabolic pathway
Evidence Blueprint
Generation and characterization of mature hepatocyte organoids for liver metabolic studies
![](https://psychiatryai.com/wp-content/uploads/2023/04/psychiatryai_com.webp)
☊ AI-Driven Related Evidence Nodes
(recent articles with at least 5 words in title)
More Evidence
![](https://psychiatryai.com/wp-content/uploads/2023/04/psychiatryai_com.webp)
Generation and characterization of mature hepatocyte organoids for liver metabolic studies
🌐 365 Days
AI Virtual Reality Related Evidence Matrix
Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids Humanized mouse liver reveals endothelial control of essential hepatic metabolic functions Mass Spectrometry Reveals that Oxysterols are Secreted from Non-Alcoholic Fatty Liver Disease Induced Organoids Dexamethasone exposure during pregnancy triggers metabolic syndrome in offspring via epigenetic alteration of IGF1 Regulatory pathways and therapeutic potential of PDE4 in liver pathophysiology Research progress of celastrol on the prevention and treatment of metabolic associated fatty liver disease Hepatic extracellular ATP/adenosine dynamics in zebrafish models of alcoholic and metabolic steatotic liver disease A novel experimental model of MetALD in male mice recapitulates key features of severe alcohol-associated hepatitis Metabolic changes and propensity for inflammation, fibrosis, and cancer in livers of mice lacking lysosomal acid lipase Inflammatory liver diseases and susceptibility to sepsis Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells Human resident liver myeloid cells protect against metabolic stress in obesity TET3 boosts hepatocyte autophagy and impairs non-alcoholic fatty liver disease by increasing ENPP1 promoter hypomethylation Establishment and bioinformatics evaluation of the ethanol combined with palmitic acid-induced mouse hepatocyte AFLD model (the Hu-Qiu Model) Increased Risk of Colorectal Adenomas with Metabolic-Associated Fatty Liver Disease Components Inhibition of free heme-catalyzed Fenton-like reaction prevents non-alcoholic fatty liver disease by hepatocyte-targeted hydrogen delivery Plant miR8126-3p and miR8126-5p Decrease Lipid Accumulation through Modulation of Metabolic Genes in a Human Hepatocyte Model That Mimics Steatosis Robinetin Alleviates Metabolic Failure in Liver through Suppression of p300-CD38 Axis Galectin-3 and Severity of Liver Fibrosis in Metabolic Dysfunction-Associated Fatty Liver Disease Interactions between the metabolic syndrome and alcohol consumption increases the risk of liver disease Customized liver organoids as an advanced in vitro modeling and drug discovery platform for non-alcoholic fatty liver diseases Effects of In Utero PFOS Exposure on Epigenetics and Metabolism in Mouse Fetal Livers Mass spectrometry imaging-based metabolomics highlights spatial metabolic alterations in three types of liver injuries Patients with Celiac Disease Have High Prevalence of Fatty Liver and Metabolic Syndrome Metabolic Disorders in Liver Transplant Recipients: The State of the Art Amelioration of NAFLD by sleeve gastrectomy-triggered hepatocyte regeneration in mice - experimental research Therapeutic Activity of Green Tea Epigallocatechin-3-Gallate on Metabolic Diseases and Non-Alcoholic Fatty Liver Diseases: The Current Updates Role of lactate and lactate metabolism in liver diseases (Review) Liver-specific mitochondrial amidoxime-reducing component 1 (Mtarc1) knockdown protects the liver from diet-induced MASH in multiple mouse models Generation of human cerebral organoids with a structured outer subventricular zone Adverse Maternal Environments Perturb Hepatic DNA Methylome and Transcriptome Prior to the Adult-Onset Non-Alcoholic Fatty Liver Disease in Mouse Offspring Spatial genomics: mapping human steatotic liver disease ACLY as a modulator of liver cell functions and its role in Metabolic Dysfunction-Associated Steatohepatitis MBOAT7 in liver and extrahepatic diseases New nomenclature for fatty liver disease Identification and validation of cuproptosis-related molecular clusters in non-alcoholic fatty liver disease Design optimization of geometrically confined cardiac organoids enabled by machine learning techniques Multi-monoubiquitination controls VASP-mediated actin dynamics The Transition in Terminology: From Non-alcoholic Fatty Liver Disease (NAFLD) to Metabolic Dysfunction-associated Fatty Liver Disease (MAFLD) to Metabolic-associated Steatotic Liver Disease (MASLD) Therapeutic potential of oleanolic acid in liver diseases Advances in management of metabolic dysfunction-associated steatotic liver disease: from mechanisms to therapeutics Patient Brain Organoids Identify a Link between the 16p11.2 Copy Number Variant and the RBFOX1 Gene Brain organoids: A revolutionary tool for modeling neurological disorders and development of therapeutics Insights of mitochondrial involvement in alcoholic fatty liver disease Evaluation of the efficacy of mitochondrial fission inhibitor (Mdivi-1) using non-alcoholic steatohepatitis (NASH) liver organoids The Role of Phosphatidylethanolamine N-Methyltransferase (PEMT) and Its Waist-Hip-Ratio-Associated Locus rs4646404 in Obesity-Related Metabolic Traits and Liver Disease Reduced Lipopolysaccharide-Binding Protein (LBP) Levels Are Associated with Non-Alcoholic Fatty Liver Disease (NAFLD) and Adipose Inflammation in Human Obesity Identification and characterisation of a rare MTTP variant underlying hereditary non-alcoholic fatty liver disease The neuronal transcription factor MEIS2 is a calpain2 protease target Amphiregulin from regulatory T cells promotes liver fibrosis and insulin resistance in non-alcoholic steatohepatitis Pivotal role of intestinal cholesterol and nuclear receptor LXR in metabolic liver steatohepatitis and hepatocarcinoma Kupffer cell diversity maintains liver function in alcohol-associated liver disease The GPR40 novel agonist SZZ15-11 improves non-alcoholic fatty liver disease by activating the AMPK pathway and restores metabolic homeostasis in diet-induced obese mice An unbiased ranking of murine dietary models based on their proximity to human metabolic dysfunction-associated steatotic liver disease (MASLD) Coexistence of metabolic-associated fatty liver disease and autoimmune or toxic liver disease Fatty liver disease's renaming impacts on drug clinical trials The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging The role of Sirtuin 2 in liver - An extensive and complex biological process Cross-species analysis identifies mitochondrial dysregulation as a functional consequence of the schizophrenia-associated 3q29 deletion The deleterious effects and potential therapeutic strategy of fluorene-9-bisphenol on circadian activity and liver diseases in zebrafish and mice Therapeutic management of metabolic dysfunction associated steatotic liver disease Cyanotoxin cylindrospermopsin disrupts lipid homeostasis and metabolism in a 3D in vitro model of the human liver