Evidence
J Cell Sci. 2024 May 3:jcs.261961. doi: 10.1242/jcs.261961. Online ahead of print.
ABSTRACT
Hepatocyte organoids (HOs) generated in vitro recently are powerful tools for liver regeneration. However, the reported HOs were mostly fetal in nature with low expression levels of metabolic genes characteristic of adult liver functions, hampering their application in studying metabolic regulations and therapeutic testing for liver disorders. We report development of novel culture conditions that contains optimized levels of Triiodothyronine (T3) with the removal of growth factors, enabled successful generation of mature hepatocyte organoids (MHOs) with metabolic functions characteristic of adult livers of both mouse and human origins. We showed that the MHOs can be used to study various metabolic functions including bile and urea production, zonal metabolic gene expression, and metabolic alterations in both alcoholic and non-alcoholic fatty liver diseases as well hepatocyte proliferation, injury, and cell fate changes. Notably, the MHOs derived from human fetal hepatoblasts also showed improved hepatitis B virus (HBV) infection. Therefore, these MHOs provide a powerful in vitro model for studies of human liver physiology and diseases. The human MHOs are potentially a robust research tools for therapeutic development as well.
PMID:38700490 | DOI:10.1242/jcs.261961
Estimated reading time: 4 minute(s)
Latest: Psychiatryai.com #RAISR4D Evidence
Cool Evidence: Engaging Young People and Students in Real-World Evidence
Real-Time Evidence Search [Psychiatry]
AI Research
Generation and characterization of mature hepatocyte organoids for liver metabolic studies
🌐 90 Days
AI Virtual Reality Related Evidence Matrix
- Enhanced In Vitro Recapitulation of In Vivo Liver Regeneration by Co-Culturing Hepatocyte Organoids with Adipose-Derived Mesenchymal Stem Cells, Alleviating Steatosis and Apoptosis in Acute Alcoholic Liver Injury
- Enhanced In Vitro Recapitulation of In Vivo Liver Regeneration by Co-Culturing Hepatocyte Organoids with Adipose-Derived Mesenchymal Stem Cells, Alleviating Steatosis and Apoptosis in Acute Alcoholic Liver Injury
- Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids
- Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids
- A novel experimental model of MetALD in male mice recapitulates key features of severe alcohol-associated hepatitis
- Intricate interplay between cell metabolism and necroptosis regulation in metabolic dysfunction-associated steatotic liver disease: A narrative review
- Immunological mechanisms in steatotic liver diseases: An overview and clinical perspectives
- A PNPLA3-Deficient iPSC-Derived Hepatocyte Screen Identifies Pathways to Potentially Reduce Steatosis in Metabolic Dysfunction-Associated Fatty Liver Disease
- Exerkines: Benign adaptation for exercise and benefits for non-alcoholic fatty liver disease
- Role of lactate and lactate metabolism in liver diseases (Review)
- New nomenclature for fatty liver disease
- Design optimization of geometrically confined cardiac organoids enabled by machine learning techniques
- From non-alcoholic fatty liver disease to metabolic-associated steatotic liver disease: Rationale and implications for the new terminology
- Metabolic-associated fatty liver disease is less effective in predicting mortality than non-alcoholic fatty liver disease and metabolic dysfunction-associated steatotic liver disease
- The Transition in Terminology: From Non-alcoholic Fatty Liver Disease (NAFLD) to Metabolic Dysfunction-associated Fatty Liver Disease (MAFLD) to Metabolic-associated Steatotic Liver Disease (MASLD)
- Role of gut microbiota and immune cells in metabolic-associated fatty liver disease: clinical impact
- Platycodon D reduces obesity and non-alcoholic fatty liver disease induced by a high-fat diet through inhibiting intestinal fat absorption
- Pivotal role of intestinal cholesterol and nuclear receptor LXR in metabolic liver steatohepatitis and hepatocarcinoma
- Metabolic dysfunction-associated steatotic liver disease prevalence and risk factors in inflammatory bowel disease in tertiary center
- An unbiased ranking of murine dietary models based on their proximity to human metabolic dysfunction-associated steatotic liver disease (MASLD)
- Coexistence of metabolic-associated fatty liver disease and autoimmune or toxic liver disease
- The deleterious effects and potential therapeutic strategy of fluorene-9-bisphenol on circadian activity and liver diseases in zebrafish and mice
- FGL1 and FGL2: emerging regulators of liver health and disease
- Pediatric metabolic (dysfunction)-associated fatty liver disease: current insights and future perspectives
- Hericium erinaceus polysaccharides ameliorate nonalcoholic fatty liver disease via gut microbiota and tryptophan metabolism regulation in an aged laying hen model
- A glia-enriched stem cell 3D model of the human brain mimics the glial-immune neurodegenerative phenotypes of multiple sclerosis
- Spinal cord injury-induced metabolic impairment and steatohepatitis develops in non-obese rats and is exacerbated by premorbid obesity
- PSTPIP2 protects against alcoholic liver injury and invokes STAT3-mediated suppression of apoptosis
- Aging exacerbates oxidative stress and liver fibrosis in an animal model of Down Syndrome
- A male mouse model for metabolic dysfunction-associated steatotic liver disease and hepatocellular carcinoma
- Gut-liver axis: Potential mechanisms of action of food-derived extracellular vesicles
- RNA modifications in the progression of liver diseases: from fatty liver to cancer
- RNA modifications in the progression of liver diseases: from fatty liver to cancer
- Generating Neuroimmune Assembloids Using Human Induced Pluripotent Stem Cell (iPSC)-Derived Cortical Organoids and Microglia
- Cardiometabolic and Metabolic Profiles of Lean/Normal, Overweight and Obese Patients with Nonalcoholic Fatty Liver Disease
- Alcohol- and Low-Iron Induced Changes in Antioxidant and Energy Metabolism Associated with Protein Lys Acetylation
- Single-Cell Data Analysis Reveals Critical Hepatic Cells Subpopulations in the Progression of Non-alcoholic Fatty Liver Disease to Non-Alcoholic Steatohepatitis
- Monitoring disease progression in metabolic dysfunction-associated steatotic liver disease
- Monitoring disease progression in metabolic dysfunction-associated steatotic liver disease
- MAFLD in adults: non-invasive tests for diagnosis and monitoring of MAFLD
- Fecal virome transplantation: A promising strategy for the treatment of metabolic diseases
- Fecal virome transplantation: A promising strategy for the treatment of metabolic diseases
- Gentiopicroside improves NASH and liver fibrosis by suppressing TLR4 and NLRP3 signaling pathways
- Haloacetamides disinfection by-products, a potential risk factor for nonalcoholic fatty liver disease
- Guidelines for the prevention and treatment of metabolic dysfunction-associated (non-alcoholic) fatty liver disease (Version 2024)
- Guidelines for the prevention and treatment of metabolic dysfunction-associated (non-alcoholic) fatty liver disease (Version 2024)
- Multiscale 3D genome organization underlies duck fatty liver with no adipose inflammation or serious injury
- Metabolic dysfunction-associated fatty liver disease and low muscle strength: A comment
- AMPED study: Protocol for a randomized controlled trial of different doses of aerobic exercise training
- Comparative study of MAFLD as a predictor of metabolic disease treatment for NAFLD
- Pathogenic gene connections in type 2 diabetes and non-alcoholic fatty liver disease: a bioinformatics analysis and mouse model investigations experiments
- Drug Evaluation of Parkinson's Disease Patient-Derived Midbrain Organoids Using Mesoporous Au Nanodot-Patterned 3D Concave Electrode
- Outcome prediction in metabolic dysfunction-associated steatotic liver disease using stain-free digital pathological assessment
- Mitochondrial ACSS1-K635 acetylation knock-in mice exhibit altered metabolism, cell senescence, and nonalcoholic fatty liver disease
- Washed microbiota transplantation promotes homing of group 3 innate lymphoid cells to the liver via the CXCL16/CXCR6 axis: a potential treatment for metabolic-associated fatty liver disease
- Role of metabolic dysfunction and inflammation along the liver-brain axis in animal models with obesity-induced neurodegeneration
- Metabolic Dysfunction-associated Fatty Liver Disease: An Urgent Call for Global Action
- Sesaminol alters phospholipid metabolism and alleviates obesity-induced NAFLD
- Exploring Fibrosis Pathophysiology in Lean and Obese Metabolic-Associated Fatty Liver Disease: An In-Depth Comparison
- Insulin-like growth factor binding protein-3 (igfbp-3) and igfbp-5 in yellowtail kingfish (Seriola lalandi): molecular characterization and expression levels under different nutritional status and stocking density
- Breakthrough in the Treatment of Metabolic Associated Steatotic Liver Disease: Is it all over?
- Managing the Patient with Psoriasis and Metabolic Comorbidities
- Crosstalk between Epigenetics and Metabolic Reprogramming in Metabolic Dysfunction-Associated Steatotic Liver Disease-Induced Hepatocellular Carcinoma: A New Sight
- Lipid Nanoparticle-Mediated Delivery of CRISPR-Cas9 Against Rubicon Ameliorates NAFLD by Modulating CD36 Along with Glycerophospholipid Metabolism
Evidence Blueprint
Generation and characterization of mature hepatocyte organoids for liver metabolic studies
☊ AI-Driven Related Evidence Nodes
(recent articles with at least 5 words in title)
More Evidence
Generation and characterization of mature hepatocyte organoids for liver metabolic studies
🌐 365 Days
AI Virtual Reality Related Evidence Matrix
- Enhanced In Vitro Recapitulation of In Vivo Liver Regeneration by Co-Culturing Hepatocyte Organoids with Adipose-Derived Mesenchymal Stem Cells, Alleviating Steatosis and Apoptosis in Acute Alcoholic Liver Injury
- Enhanced In Vitro Recapitulation of In Vivo Liver Regeneration by Co-Culturing Hepatocyte Organoids with Adipose-Derived Mesenchymal Stem Cells, Alleviating Steatosis and Apoptosis in Acute Alcoholic Liver Injury
- Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases
- Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids
- Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids
- Dexamethasone exposure during pregnancy triggers metabolic syndrome in offspring via epigenetic alteration of IGF1
- Regulatory pathways and therapeutic potential of PDE4 in liver pathophysiology
- Research progress of celastrol on the prevention and treatment of metabolic associated fatty liver disease
- Hepatic extracellular ATP/adenosine dynamics in zebrafish models of alcoholic and metabolic steatotic liver disease
- A novel experimental model of MetALD in male mice recapitulates key features of severe alcohol-associated hepatitis
- Metabolic changes and propensity for inflammation, fibrosis, and cancer in livers of mice lacking lysosomal acid lipase
- Inflammatory liver diseases and susceptibility to sepsis
- Intricate interplay between cell metabolism and necroptosis regulation in metabolic dysfunction-associated steatotic liver disease: A narrative review
- Establishment and bioinformatics evaluation of the ethanol combined with palmitic acid-induced mouse hepatocyte AFLD model (the Hu-Qiu Model)
- TET3 boosts hepatocyte autophagy and impairs non-alcoholic fatty liver disease by increasing ENPP1 promoter hypomethylation
- Increased Risk of Colorectal Adenomas with Metabolic-Associated Fatty Liver Disease Components
- Immunological mechanisms in steatotic liver diseases: An overview and clinical perspectives
- Plant miR8126-3p and miR8126-5p Decrease Lipid Accumulation through Modulation of Metabolic Genes in a Human Hepatocyte Model That Mimics Steatosis
- Robinetin Alleviates Metabolic Failure in Liver through Suppression of p300-CD38 Axis
- Galectin-3 and Severity of Liver Fibrosis in Metabolic Dysfunction-Associated Fatty Liver Disease
- Interactions between the metabolic syndrome and alcohol consumption increases the risk of liver disease
- Effects of In Utero PFOS Exposure on Epigenetics and Metabolism in Mouse Fetal Livers
- A PNPLA3-Deficient iPSC-Derived Hepatocyte Screen Identifies Pathways to Potentially Reduce Steatosis in Metabolic Dysfunction-Associated Fatty Liver Disease
- Amelioration of NAFLD by sleeve gastrectomy-triggered hepatocyte regeneration in mice - experimental research
- Metabolic Disorders in Liver Transplant Recipients: The State of the Art
- Patients with Celiac Disease Have High Prevalence of Fatty Liver and Metabolic Syndrome
- Mass spectrometry imaging-based metabolomics highlights spatial metabolic alterations in three types of liver injuries
- Exerkines: Benign adaptation for exercise and benefits for non-alcoholic fatty liver disease
- Liver-specific mitochondrial amidoxime-reducing component 1 (Mtarc1) knockdown protects the liver from diet-induced MASH in multiple mouse models
- Role of lactate and lactate metabolism in liver diseases (Review)
- Generation of human cerebral organoids with a structured outer subventricular zone
- ACLY as a modulator of liver cell functions and its role in Metabolic Dysfunction-Associated Steatohepatitis
- Spatial genomics: mapping human steatotic liver disease
- MBOAT7 in liver and extrahepatic diseases
- New nomenclature for fatty liver disease
- Identification and validation of cuproptosis-related molecular clusters in non-alcoholic fatty liver disease
- Multi-monoubiquitination controls VASP-mediated actin dynamics
- Design optimization of geometrically confined cardiac organoids enabled by machine learning techniques
- From non-alcoholic fatty liver disease to metabolic-associated steatotic liver disease: Rationale and implications for the new terminology
- Metabolic-associated fatty liver disease is less effective in predicting mortality than non-alcoholic fatty liver disease and metabolic dysfunction-associated steatotic liver disease
- Therapeutic potential of oleanolic acid in liver diseases
- Patient Brain Organoids Identify a Link between the 16p11.2 Copy Number Variant and the RBFOX1 Gene
- The Transition in Terminology: From Non-alcoholic Fatty Liver Disease (NAFLD) to Metabolic Dysfunction-associated Fatty Liver Disease (MAFLD) to Metabolic-associated Steatotic Liver Disease (MASLD)
- Advances in management of metabolic dysfunction-associated steatotic liver disease: from mechanisms to therapeutics
- Role of gut microbiota and immune cells in metabolic-associated fatty liver disease: clinical impact
- Brain organoids: A revolutionary tool for modeling neurological disorders and development of therapeutics
- Evaluation of the efficacy of mitochondrial fission inhibitor (Mdivi-1) using non-alcoholic steatohepatitis (NASH) liver organoids
- Insights of mitochondrial involvement in alcoholic fatty liver disease
- Platycodon D reduces obesity and non-alcoholic fatty liver disease induced by a high-fat diet through inhibiting intestinal fat absorption
- The Role of Phosphatidylethanolamine N-Methyltransferase (PEMT) and Its Waist-Hip-Ratio-Associated Locus rs4646404 in Obesity-Related Metabolic Traits and Liver Disease
- Reduced Lipopolysaccharide-Binding Protein (LBP) Levels Are Associated with Non-Alcoholic Fatty Liver Disease (NAFLD) and Adipose Inflammation in Human Obesity
- The neuronal transcription factor MEIS2 is a calpain2 protease target
- Kupffer cell diversity maintains liver function in alcohol-associated liver disease
- Pivotal role of intestinal cholesterol and nuclear receptor LXR in metabolic liver steatohepatitis and hepatocarcinoma
- Amphiregulin from regulatory T cells promotes liver fibrosis and insulin resistance in non-alcoholic steatohepatitis
- The GPR40 novel agonist SZZ15-11 improves non-alcoholic fatty liver disease by activating the AMPK pathway and restores metabolic homeostasis in diet-induced obese mice
- Fatty liver disease's renaming impacts on drug clinical trials
- Cross-species analysis identifies mitochondrial dysregulation as a functional consequence of the schizophrenia-associated 3q29 deletion
- The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging
- Metabolic dysfunction-associated steatotic liver disease prevalence and risk factors in inflammatory bowel disease in tertiary center
- An unbiased ranking of murine dietary models based on their proximity to human metabolic dysfunction-associated steatotic liver disease (MASLD)
- Coexistence of metabolic-associated fatty liver disease and autoimmune or toxic liver disease
- The deleterious effects and potential therapeutic strategy of fluorene-9-bisphenol on circadian activity and liver diseases in zebrafish and mice
- The role of Sirtuin 2 in liver - An extensive and complex biological process