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Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

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STAR Protoc. 2024 Apr 27;5(2):103027. doi: 10.1016/j.xpro.2024.103027. Online ahead of print.

ABSTRACT

Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head-fixed recordings. For complete details on the use and execution of this protocol, please refer to Dadarlat et al.1.

PMID:38678569 | DOI:10.1016/j.xpro.2024.103027

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Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

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Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

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Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

Evidence

STAR Protoc. 2024 Apr 27;5(2):103027. doi: 10.1016/j.xpro.2024.103027. Online ahead of print.

ABSTRACT

Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head-fixed recordings. For complete details on the use and execution of this protocol, please refer to Dadarlat et al.1.

PMID:38678569 | DOI:10.1016/j.xpro.2024.103027

Document this CPD Copy URL Button

Google

Google Keep

LinkedIn Share Share on Linkedin

Estimated reading time: 2 minute(s)

Latest: Psychiatryai.com #RAISR4D Evidence

Cool Evidence: Engaging Young People and Students in Real-World Evidence

Real-Time Evidence Search [Psychiatry]

AI Research

Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

Copy WordPress Title

🌐 90 Days

Evidence Blueprint

Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

QR Code

☊ AI-Driven Related Evidence Nodes

(recent articles with at least 5 words in title)

More Evidence

Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

🌐 365 Days

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