Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging
Evidence
STAR Protoc. 2024 Apr 27;5(2):103027. doi: 10.1016/j.xpro.2024.103027. Online ahead of print.
ABSTRACT
Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head-fixed recordings. For complete details on the use and execution of this protocol, please refer to Dadarlat et al.1.
Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging
Evidence
STAR Protoc. 2024 Apr 27;5(2):103027. doi: 10.1016/j.xpro.2024.103027. Online ahead of print.
ABSTRACT
Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head-fixed recordings. For complete details on the use and execution of this protocol, please refer to Dadarlat et al.1.
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