Evidence
J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Jul 7;1228:123824. doi: 10.1016/j.jchromb.2023.123824. Online ahead of print.
ABSTRACT
In the present study an enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the first time for quantitative determination of the recreational drug of abuse methylone and its major metabolites in oral fluid. The simultaneous chemo- and enantioseparation of methylone and its major metabolites was performed on a polysaccharide-based chiral column based on amylose tris(5-chloro-3-methylphenylcarbamate) as chiral selector (Lux i-Amylose-3) with methanol containing 0.4 % (v/v) aqueous ammonium hydroxide as mobile phase. The time required for enantioselective analysis of methylone and its 2 major metabolites was 15 min. This method was fully validated following the Organization of Scientific Area Committees (OSAC) for Forensic Science guidelines. This method was applied for the enantioselective determination of methylone and its metabolites in oral fluid and enantioselectivity in metabolism and pharmacokinetic of the parent compound and metabolites was observed. While the first enantiomer of methylone was found at higher concentration, both metabolites shown greater concentration for the second enantiomer. The results revealed that MET undergoes an enantioselective biotransformation to its metabolites HMMC and MDC, with S-(-)-MET more rapidly metabolized and eliminated from the body.
PMID:37487291 | DOI:10.1016/j.jchromb.2023.123824
Estimated reading time: 4 minute(s)
Latest: Psychiatryai.com #RAISR4D Evidence
Cool Evidence: Engaging Young People and Students in Real-World Evidence
Real-Time Evidence Search [Psychiatry]
AI Research
Development of enantioselective high-performance liquid chromatography-tandem mass spectrometry method for quantitative determination of methylone and some of its metabolites in oral fluid
🌐 90 Days
AI Virtual Reality Related Evidence Matrix
- Multifunctional-separation-mode ion chromatography method for determining major metabolites during multiple parallel fermentation of rice wine
- Multifunctional-separation-mode ion chromatography method for determining major metabolites during multiple parallel fermentation of rice wine
- N-ethylhexedrone: a very long and bad trip! A Case series
- Phytosterols in human serum as measured using a liquid chromatography tandem mass spectrometry
- Simultaneous mass spectrometric quantification of trace amines, their precursors and metabolites
- Advanced UPLC-MS/MS Method for the Quantification of SIPI6398 in Rat Plasma and Its Pharmacokinetic Characterization
- Determination of lamotrigine in human plasma by HPLC-PDA. Application to forensic samples
- Neuroforensomics: metabolites as valuable biomarkers in cerebrospinal fluid of lethal traumatic brain injuries
- A UPLC-MS/MS method for simultaneous determination of arachidonic acid, stearic acid, and related endocannabinoids in human plasma
- Determination of 10 mycotoxins in wine, baijiu, and huangjiu of the Chinese market by liquid chromatography tandem mass spectrometry and exposure estimation
- Study on the pharmacokinetics, tissue distribution and excretion of Penthorum chinense Pursh in normal and acute alcoholic liver injury rats using validated UPLC-MS/MS method
- Phytochemical Profiling and Biological Activities of Pericarps and Seeds Reveal the Controversy on "Enucleation" or "Nucleus-Retaining" of Cornus officinalis Fruits
- Mag-Net: Rapid enrichment of membrane-bound particles enables high coverage quantitative analysis of the plasma proteome
- Serum metabolomics analysis combined with network pharmacology reveals possible mechanisms of postoperative cognitive dysfunction in the treatment of Mongolian medicine Eerdun Wurile basic formula
- Targeted metabolomics-based understanding of the sleep disturbances in drug-naive patients with schizophrenia
- Rapid Screening of New Psychoactive Substances Using pDART-QqQ-MS
- Cerebrospinal fluid metabolomes of treatment-resistant depression subtypes and ketamine response: a pilot study
- Serum neuroactive metabolites of the tryptophan pathway in patients with acute phase of affective disorders
- Mangiferin alleviated poststroke cognitive impairment by modulating lipid metabolism in cerebral ischemia/reperfusion rats
- Association of the retinol to all-trans retinoic acid pathway with autism spectrum disorder
- Unveiling the altered metabolic pathways induced by nivolumab in non-small cell lung cancer via GC-MS metabolomics approach coupled with multivariate analysis
- Nitrogen-doped magnetic porous carbon nanospheres derived from dual templates-induced mesoporous polydopamine coated Fe3O4 for efficient extraction and sensitive determination of volatile nitrosamines by gas chromatography-mass spectroscopy
- Amino acid metabolomics and machine learning for assessment of post-hepatectomy liver regeneration
- Accumulation of Non-Pathological Liver Fat Is Associated with the Loss of Glyoxalase I Activity in Humans
- Chromatographic Methods for the Analysis of the Antipsychotic Drug Clozapine and Its Major Metabolites: A Review
- Continuous evaluation of single-dose moxifloxacin concentrations in brain extracellular fluid, cerebrospinal fluid, and plasma: a novel porcine model
- Rapid detection of furanyl fentanyl in complex matrices using Leidenfrost desorption-assisted low-temperature arc plasma ionization mass spectrometry
- Direct Extraction and Determination of Free Nicotine in Cigarette Smoke
- AI-assisted mass spectrometry imaging with in situ image segmentation for subcellular metabolomics analysis
- Mechanism of Xing 9 Ling tablet candy for alcoholic liver disease based on network pharmacology
- Trimethylamine-N-Oxide and Related Metabolites: Assessing Cardiovascular Risk in the Dallas Heart Study
- Trimethylamine-N-Oxide and Related Metabolites: Assessing Cardiovascular Risk in the Dallas Heart Study
- Melamine sponges incorporated azo-linked porous organic polymer as adsorbent for extraction and determination of six B vitamins using pipette tip micro solid-phase extraction
- Coprococcus eutactus screened from healthy adolescent attenuates chronic restraint stress-induced depression-like changes in adolescent mice: Potential roles in the microbiome and neurotransmitter modulation
- Coprococcus eutactus screened from healthy adolescent attenuates chronic restraint stress-induced depression-like changes in adolescent mice: Potential roles in the microbiome and neurotransmitter modulation
- Coprococcus eutactus screened from healthy adolescent attenuates chronic restraint stress-induced depression-like changes in adolescent mice: Potential roles in the microbiome and neurotransmitter modulation
- The efficacy of intermittent hemodialysis in severe bromovalerylurea poisoning
- Identification of post-mortem product of zolpidem degradation by hemoglobin via the Fenton reaction
- N-oleoyl alanine attenuates nicotine reward and spontaneous nicotine withdrawal in mice
- Circularly polarized blue fluorescence based on chiral heteroleptic six-coordinate bis-pyrazolonate-Zn2+ complexes
- Higher paracetamol levels are associated with elevated glucocorticoid concentrations in hair: findings from a large cohort of young adults
- Effects of tetramethylpyrazine on pharmacokinetics in plasma and brain dialysate and neuropathic pain in rat model of spared sciatic nerve injury
- The identification of novel schizophrenia-related metabolites using untargeted lipidomics
- Association between Per- and poly-fluoroalkyl substances and nonalcoholic fatty liver disease: a nested case-control study in northwest China
- Proteomic Analysis of Spatial Heterogeneity Identifies HMGB2 as Putative Biomarker of Tumor Progression in Adult-Type Diffuse Astrocytomas
- Bioassay-guided isolation of anti-inflammatory and antinociceptive metabolites among three Moroccan Juniperus leaves extract supported with in vitro enzyme inhibitory assays
- Multiomics analysis to explore blood metabolite biomarkers in an Alzheimer's Disease Neuroimaging Initiative cohort
- Online quantification of nicotine in e-cigarette aerosols by vacuum ultraviolet photoionization mass spectrometry
- Formation of multiple ion types during MALDI imaging mass spectrometry analysis of Mitragyna speciosa alkaloids in dosed rat brain tissue
- Metabolism, Disposition, Excretion, and Potential Transporter Inhibition of 7-16, an Improving 5-HT2A Receptor Antagonist and Inverse Agonist for Parkinson's Disease
- Alternative routine for reporting chiral amphetamine test results in assessment of attention-deficit/hyperactivity disorder medication: experiences from 2013 to 2023
- From inspection to analysis: A combined approach to identifying counterfeit whiskeys using HS-GC-FID and bottle integrity
- Pseudotargeted lipidomics analysis of scoparone on glycerophospholipid metabolism in non-alcoholic steatohepatitis mice by LC-MRM-MS
- The metabolome-wide signature of major depressive disorder
- Oxidatively-induced DNA base damage and base excision repair abnormalities in siblings of individuals with bipolar disorder DNA damage and repair in bipolar disorder
- Localized Pantothenic Acid (Vitamin B5) Reductions Present Throughout the Dementia with Lewy Bodies Brain
- Survival estimation of oral cancer using fuzzy deep learning
- A high-precision view of intercompartmental drug transport via simultaneous, seconds-resolved, in situ measurements in the vein and brain
- A high-precision view of intercompartmental drug transport via simultaneous, seconds-resolved, in situ measurements in the vein and brain
- In vitro study of the blood-brain barrier transport of bioactives from Mediterranean foods
- High-Efficiency Effect-Directed Analysis Leveraging Five High Level Advancements: A Critical Review
- eLIMS: Ensemble Learning-Based Spatial Segmentation of Mass Spectrometry Imaging to Explore Metabolic Heterogeneity
- Local Structural Features Elucidate Crystallization of Complex Structures
- Application of omics in the diagnosis, prognosis, and treatment of acute myeloid leukemia
Evidence Blueprint
Development of enantioselective high-performance liquid chromatography-tandem mass spectrometry method for quantitative determination of methylone and some of its metabolites in oral fluid
☊ AI-Driven Related Evidence Nodes
(recent articles with at least 5 words in title)
More Evidence
Development of enantioselective high-performance liquid chromatography-tandem mass spectrometry method for quantitative determination of methylone and some of its metabolites in oral fluid
🌐 365 Days
AI Virtual Reality Related Evidence Matrix
- Chiral analysis and semi-preparative separation of metconazole stereoisomers by supercritical fluid chromatography and cytotoxicity assessment in vitro
- Multifunctional-separation-mode ion chromatography method for determining major metabolites during multiple parallel fermentation of rice wine
- Multifunctional-separation-mode ion chromatography method for determining major metabolites during multiple parallel fermentation of rice wine
- Serum metabolomics study of narcolepsy type 1 based on ultra-performance liquid chromatography-tandem mass spectrometry
- N-ethylhexedrone: a very long and bad trip! A Case series
- Simultaneous quantification of 2-aminothiazoline-4-carboxylic acid and 2-aminothiazoline-4-oxoaminoethanoic acid utilizing chemical derivatization followed by liquid chromatography-tandem mass spectrometry
- Phytosterols in human serum as measured using a liquid chromatography tandem mass spectrometry
- Chemical profiling and quality evaluation of Liuweizhiji Gegen-Sangshen oral liquid by UPLC-Q-TOF-MS and HPLC-diode array detector fingerprinting
- A fluorescence chemo sensor approach for determination of finerenone in pharmaceutical formulation and human plasma: Method development and validation
- Simultaneous mass spectrometric quantification of trace amines, their precursors and metabolites
- Determination of nicotine and cotinine in human hair using online solid phase extraction coupled with liquid chromatography-tandem mass spectrometry and their relation to hair cortisol and cortisone
- Targeted quantitative metabolic profiling of brain-derived cell cultures by semi-automated MEPS and LC-MS/MS
- Simultaneous Determination of Prednisone and Prednisolone in Serum by Turbulent Flow Liquid Chromatography-Tandem Mass Spectrometry
- Advanced UPLC-MS/MS Method for the Quantification of SIPI6398 in Rat Plasma and Its Pharmacokinetic Characterization
- Determination of lamotrigine in human plasma by HPLC-PDA. Application to forensic samples
- Development of a carbamazepine determination method based on high-performance liquid chromatography with diode array
- Functionalized magnetic metal organic framework nanocomposites for high throughput automation extraction and sensitive detection of antipsychotic drugs in serum samples
- Patients with Neurodegenerative Proteinopathies Exhibit Altered Tryptophan Metabolism in the Serum and Cerebrospinal Fluid
- Investigating alcohol consumption in China via wastewater-based epidemiology
- Quantification of fluticasone propionate in human plasma by LC-MS/MS and its application in the pharmacokinetic study of nasal spray at clinical doses
- Detection of thiocyanate in postmortem blood of a fatal potassium thiocyanate-poisoning patient using gas chromatography-mass spectrometry
- Methylone is a rapid-acting neuroplastogen with less off-target activity than MDMA
- Identification and quantification of (poly)phenol and methylxanthine metabolites in human cerebrospinal fluid: evidence of their ability to cross the BBB
- Neuroforensomics: metabolites as valuable biomarkers in cerebrospinal fluid of lethal traumatic brain injuries
- A UPLC-MS/MS method for simultaneous determination of arachidonic acid, stearic acid, and related endocannabinoids in human plasma
- Detection of major psychoactive compounds (safrole, myristicin, and elemicin) of nutmeg in human serum via GC-MS/MS using MonoSpin® extraction: Application in a nutmeg poisoning case
- Determination of 10 mycotoxins in wine, baijiu, and huangjiu of the Chinese market by liquid chromatography tandem mass spectrometry and exposure estimation
- Study on the pharmacokinetics, tissue distribution and excretion of Penthorum chinense Pursh in normal and acute alcoholic liver injury rats using validated UPLC-MS/MS method
- Residual behavior of dinotefuran and its metabolites during Huangjiu fermentation and their effects on flavor
- Development and validation for bioanalysis of VK2809, its active metabolite VK2809A and glutathione-conjugated metabolite MB06588 in rat liver using LC-MS/MS
- Phytochemical Profiling and Biological Activities of Pericarps and Seeds Reveal the Controversy on "Enucleation" or "Nucleus-Retaining" of Cornus officinalis Fruits
- Mag-Net: Rapid enrichment of membrane-bound particles enables high coverage quantitative analysis of the plasma proteome
- The contribution of polyamine pathway to determinations of diagnosis for treatment-resistant depression: A metabolomic analysis
- Metabolic profiling of Alzheimer's disease: Untargeted metabolomics analysis of plasma samples
- Algorithm for rapid exclusion of clinically relevant plasma levels of DOACs in patients using the DOAC Dipstick. An expert consensus paper
- Serum metabolomics analysis combined with network pharmacology reveals possible mechanisms of postoperative cognitive dysfunction in the treatment of Mongolian medicine Eerdun Wurile basic formula
- Disposition of Hexahydrocannabinol Epimers and Their Metabolites in Biological Matrices following a Single Administration of Smoked Hexahydrocannabinol: A Preliminary Study
- Metabolic profile of N-ethylhexedrone, N-ethylpentedrone, and 4-chloromethcathinone in urine samples by UHPLC-QTOF-HRMS
- Untargeted metabolomics analysis in drug-naïve patients with severe obsessive-compulsive disorder
- Quantitation of Urine Nicotine, Cotinine, and 3-OH-Cotinine by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
- Analysis of dipeptides in Chinese liquors based on dansylation combined with liquid chromatography-mass spectrometry
- Cord Blood Metabolite Profiles and Their Association with Autistic Traits in Childhood
- Prevalence and drug use correlates of inadvertent fentanyl exposure among individuals misusing drugs in seven U.S. states
- Targeted metabolomics-based understanding of the sleep disturbances in drug-naive patients with schizophrenia
- An analytical approach to determining pethidine: An investigation of 18 patients' urine
- Rapid detection of four amphetamine-type drugs in hair by pulsed direct current electrospray mass spectrometry
- Behavioral metabolomics: how behavioral data can guide metabolomics research on neuropsychiatric disorders
- Rapid Screening of New Psychoactive Substances Using pDART-QqQ-MS
- New aspects characterizing non-obese NAFLD by the analysis of the intestinal flora and metabolites using a mouse model
- Cerebrospinal fluid metabolomes of treatment-resistant depression subtypes and ketamine response: a pilot study
- Exploiting the potential of fabric phase sorptive extraction for forensic food safety: Analysis of food samples in cases of drug facilitated crimes
- Serum neuroactive metabolites of the tryptophan pathway in patients with acute phase of affective disorders
- The genomic alterations in glioblastoma influence the levels of CSF metabolites
- The protective effect of Enteromorpha prolifera polysaccharide on alcoholic liver injury in C57BL/6 mice
- Mangiferin alleviated poststroke cognitive impairment by modulating lipid metabolism in cerebral ischemia/reperfusion rats
- Identifying Sex-Specific Serum Patterns of Alzheimer's Mice through Deep TMT Profiling and a Concentration-Dependent Concatenation Strategy
- Terpenes based hydrophobic deep eutectic solvents for dispersive liquid-liquid microextraction of aliphatic aldehydes in drinking water and alcoholic beverages
- The Urine Metabolome of R6/2 and zQ175DN Huntington's Disease Mouse Models
- Association of the retinol to all-trans retinoic acid pathway with autism spectrum disorder
- Evaluation of organophosphorus pesticide tyrosine adducts for post-mortem change by human serum albumin with liquid chromatography quadrupole orbitrap mass spectrometry
- Determination of Methanol, Acetaldehyde, and Ethyl Acetate in Thousand Folds of Ethanol Sample by Headspace Gas Chromatography with Mass Spectrometry
- The first human normative ranges and biomarker performance of dimethylguanidino valeric acid isoforms in fatty liver disease
- Fndc5/Irisin Deficiency Leads to Dysbiosis of Gut Microbiota Contributing to the Depressive-like Behaviors in Mice
- Associations between Plasma Lipid Mediators and Chronic Daily Headache Outcomes in Patients Randomized to a Low Linoleic Acid Diet with or without Added Omega-3 Fatty Acids