Effects of hypoxia in the diabetic corneal stroma microenvironment
Evidence
Exp Eye Res. 2024 Jan 13:109790. doi: 10.1016/j.exer.2024.109790. Online ahead of print.
ABSTRACT
Corneal dysfunctions associated with Diabetes Mellitus (DM), termed diabetic keratopathy (DK), can cause impaired vision and/or blindness. Hypoxia affects both Type 1 (T1DM) and Type 2 (T2DM) surprisingly, the role of hypoxia in DK is unexplored. The aim of this study was to examine the impact of hypoxia in vitro on primary human corneal stromal cells derived from Healthy (HCFs), and diabetic (T1DMs and T2DMs) subjects, by exposing them to normoxic (21% O2) or hypoxic (2% O2) conditions through 2D and 3D in vitro models. Our data revealed that hypoxia affected T2DMs by slowing their wound healing capacity, leading to significant alterations in oxidative stress-related markers, mitochondrial health, cellular homeostasis, and endoplasmic reticulum health (ER) along with fibrotic development. In T1DMs, hypoxia significantly modulated markers related to membrane permeabilization, oxidative stress via apoptotic marker (BAX), and protein degradation. Hypoxic environment induced oxidative stress (NOQ1 mediated reduction of superoxide in T1DMs and Nrf2 mediated oxidative stress in T2DMs), modulation in mitochondrial health (Heat shock protein 27 (HSP27), and dysregulation of cellular homeostasis (HSP90) in both T1DMs and T2DMs. This data underscores the significant impact of hypoxia on the diabetic cornea. Further studies are warranted to delineate the complex interactions.
BMC Prim Care. 2024 Jun 21;25(1):223. doi: 10.1186/s12875-024-02447-9.ABSTRACTBACKGROUND: The caretaking process for older adults with depression and physical multimorbidity is complex. Older patients with both...
BMC Prim Care. 2024 Jun 21;25(1):223. doi: 10.1186/s12875-024-02447-9.ABSTRACTBACKGROUND: The caretaking process for older adults with depression and physical multimorbidity is complex. Older patients with both...
Trials. 2024 Jun 22;25(1):408. doi: 10.1186/s13063-024-08238-y.ABSTRACTBACKGROUND: There are no approved pharmacotherapies for methamphetamine use disorder. Two preliminary phase 2 randomised controlled trials have found mirtazapine,...
Trials. 2024 Jun 22;25(1):408. doi: 10.1186/s13063-024-08238-y.ABSTRACTBACKGROUND: There are no approved pharmacotherapies for methamphetamine use disorder. Two preliminary phase 2 randomised controlled trials have found mirtazapine,...
Ann Gen Psychiatry. 2024 Jun 21;23(1):22. doi: 10.1186/s12991-024-00510-4.ABSTRACTPURPOSE: This study aimed to assess the incidence of EEG abnormalities (EEG-ab) in children diagnosed with ADHD, investigate...
BMC Psychiatry. 2024 Jun 21;24(1):464. doi: 10.1186/s12888-024-05913-x.ABSTRACTBACKGROUND: The prevalence of hazardous substance use is highest in the age between 18 and 25, but few young...
This website uses cookies to improve your experience. We'll assume you're ok with this, but you can opt-out if you wish. Cookie settingsACCEPT
Privacy & Cookies Policy
Privacy Overview
This website uses cookies to improve your experience while you navigate through the website. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may have an effect on your browsing experience.
Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.
Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.