Evidence
Front Cell Neurosci. 2023 May 5;17:1106287. doi: 10.3389/fncel.2023.1106287. eCollection 2023.
ABSTRACT
Neurodegenerative diseases remain incompletely understood and therapies are needed. Stem cell-derived organoid models facilitate fundamental and translational medicine research. However, to which extent differential neuronal and glial pathologic processes can be reproduced in current systems is still unclear. Here, we tested 16 different chemical, physical, and cell functional manipulations in mouse retina organoids to further explore this. Some of the treatments induce differential phenotypes, indicating that organoids are competent to reproduce distinct pathologic processes. Notably, mouse retina organoids even reproduce a complex pathology phenotype with combined photoreceptor neurodegeneration and glial pathologies upon combined (not single) application of HBEGF and TNF, two factors previously associated with neurodegenerative diseases. Pharmacological inhibitors for MAPK signaling completely prevent photoreceptor and glial pathologies, while inhibitors for Rho/ROCK, NFkB, and CDK4 differentially affect them. In conclusion, mouse retina organoids facilitate reproduction of distinct and complex pathologies, mechanistic access, insights for further organoid optimization, and modeling of differential phenotypes for future applications in fundamental and translational medicine research.
PMID:37213216 | PMC:PMC10196395 | DOI:10.3389/fncel.2023.1106287
Estimated reading time: 3 minute(s)
Latest: Psychiatryai.com #RAISR4D
Cool Evidence: Engaging Young People and Students in Real-World Evidence
Real-Time Evidence Search [Psychiatry]
AI Research
Modeling inducible neuropathologies of the retina with differential phenotypes in organoids
🌐 90 Days
VR Related Evidence Matrix
- APOE3 Christchurch modulates β-catenin/Wnt signaling in iPS cell-derived cerebral organoids from Alzheimer's cases
- A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
- Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases
- Altered neuroepithelial morphogenesis and migration defects in iPSC-derived cerebral organoids and 2D neural stem cells in familial bipolar disorder
- Induced Pluripotent Stem Cells and Organoids in Advancing Neuropathology Research and Therapies
- Morphological and electrophysiological characterization of a novel displaced astrocyte in the mouse retina
- Generation of human cerebral organoids with a structured outer subventricular zone
- The NAD+ Precursor Nicotinamide Riboside Rescues Mitochondrial Defects and Neuronal Loss in iPSC derived Cortical Organoid of Alpers' Disease
- Generation and characterization of mature hepatocyte organoids for liver metabolic studies
- iPSC-derived models for anorexia nervosa research
- Stem cell modeling of nervous system tumors
- ARID1B controls transcriptional programs of axon projection in an organoid model of the human corpus callosum
- Extracellular vesicles secreted by 3D tumor organoids are enriched for immune regulatory signaling biomolecules compared to conventional 2D glioblastoma cell systems
- Differential expression analysis identifies candidate synaptogenic molecules for wiring direction-selective circuits in the retina
- Characterization of enhancer activity in early human neurodevelopment using Massively Parallel Reporter Assay (MPRA) and forebrain organoids
- Identifying Hmga2 preserving visual function by promoting a shift of Müller glia cell fate in mice with acute retinal injury
- Identifying Hmga2 preserving visual function by promoting a shift of Müller glia cell fate in mice with acute retinal injury
- Osteopontin drives neuroinflammation and cell loss in MAPT-N279K frontotemporal dementia patient neurons
- Distinct ultrastructural phenotypes of glial and neuronal alpha-synuclein inclusions in multiple system atrophy
- Human cytomegalovirus (CMV) dysregulates neurodevelopmental pathways in cerebral organoids
- The proteomic landscape of microglia in health and disease
- Neural conditional ablation of the protein tyrosine phosphatase receptor Delta PTPRD impairs gliogenesis in the developing mouse brain cortex
- Spermatogenesis: a paradigm of stem cell regulation
- Organoid bioinks: construction and application
- Lineage Analysis and Birthdating of Drosophila Central Complex Lineages
- Presynaptic Proteins and Their Roles in Visual Processing by the Retina
- Modeling HIV-1 infection and NeuroHIV in hiPSCs-derived cerebral organoid cultures
- GFAP-isoforms in the nervous system: Understanding the need for diversity
- β-amyloid accumulation enhances microtubule associated protein tau pathology in an APPNL-G-F/MAPTP301S mouse model of Alzheimer's disease
- Differential disruptions in population coding along the dorsal-ventral axis of CA1 in the APP/PS1 mouse model of Ab pathology
- Shared inflammatory glial cell signature after stab wound injury, revealed by spatial, temporal, and cell-type-specific profiling of the murine cerebral cortex
- Good Manufacturing Practice-compliant human induced pluripotent stem cells: from bench to putative clinical products
- Regulated cell death and its role in Alzheimer's disease and amyotrophic lateral sclerosis
- Ependymal cells undergo astrocyte-like reactivity in response to neuroinflammation
- Neuronal cell cycle reentry events in the aging brain are more prevalent in neurodegeneration and lead to cellular senescence
- Small-molecule-induced epigenetic rejuvenation promotes SREBP condensation and overcomes barriers to CNS myelin regeneration
- Ocular A-to-I RNA editing signatures associated with SARS-CoV-2 infection
- Exosome lncRNA IFNG-AS1 derived from mesenchymal stem cells of human adipose ameliorates neurogenesis and ASD-like behavior in BTBR mice
- Clinical, experimental and pathophysiological effects of Yaq-001: a non-absorbab le, gut-restricted adsorbent in models and patients with cirrhosis
- Defining morphologically and genetically distinct GAGBergic/cholinergic amacrine cell subtypes in the vertebrate retina
- Transcriptional programs mediating neuronal toxicity and altered glial-neuronal signaling in a Drosophila knock-in tauopathy model
- Blood vessel organoids generated by base editing and harboring single nucleotide variation in Notch3 effectively recapitulate CADASIL-related pathogenesis
- How to differentiate induced pluripotent stem cells into sensory neurons for disease modelling: a functional assessment
- Epigenetic alterations in creatine transporter deficiency: a new marker for dodecyl creatine ester therapeutic efficacy monitoring
- PolyGR and polyPR knock-in mice reveal a conserved neuroprotective extracellular matrix signature in C9orf72 ALS/FTD neurons
- Soluble and insoluble lysates from the human A53T mutant α-synuclein transgenic mouse model induces α-synucleinopathy independent of injection site
- Zebrafish raptor mutation inhibits the activity of mTORC1, inducing craniofacial defects due to autophagy-induced neural crest cell death
- Neutrophil-inflicted vasculature damage suppresses immune-mediated optic nerve regeneration
- Expression patterns of Piezo1 in the developing mouse forebrain
- Genes related to neurotransmitter receptors as potential biomarkers for Alzheimer's disease
- Targeting the EGFR pathway: An alternative strategy for the treatment of tuberous sclerosis complex?
- Trans Species RNA Activity: Sperm RNA of the Father of an Autistic Child Programs Glial Cells and Behavioral Disorders in Mice
- Editorial: Rising stars in cellular neuropathology 2022
- 5-HT1F receptor agonism induces mitochondrial biogenesis and increases cellular function in brain microvascular endothelial cells
- Understanding and manipulating the T-cell response to peptide-MHC
- Recent insights from non-mammalian models of brain injuries: an emerging literature
- Transformation of motion pattern selectivity from retina to superior colliculus
- GABAergic/glycinergic and glutamatergic neurons mediate distinct neurodevelopmental phenotypes of STXBP1 encephalopathy
- Microglial phagolysosome dysfunction and altered neural communication amplify phenotypic severity in Prader-Willi Syndrome with larger deletion
- The 37TrillionCells initiative for improving global healthcare via cell-based interception and precision medicine: focus on neurodegenerative diseases
- An update on stem cell therapy for stroke patients: Where are we now?
- Glial Cell Development and Function in the Zebrafish Central Nervous System
- Distinctive whole-brain cell types predict tissue damage patterns in thirteen neurodegenerative conditions
- Haploinsufficiency of phosphodiesterase 10A activates PI3K/AKT signaling independent of PTEN to induce an aggressive glioma phenotype
Evidence Blueprint
Modeling inducible neuropathologies of the retina with differential phenotypes in organoids
☊ AI-Driven Related Evidence Nodes
(recent articles with at least 5 words in title)
More Evidence
Modeling inducible neuropathologies of the retina with differential phenotypes in organoids
🌐 365 Days
VR Related Evidence Matrix
- Protocol for generating in vitro glioma models using human-induced pluripotent- or embryonic-stem-cell-derived cerebral organoids
- Patient Brain Organoids Identify a Link between the 16p11.2 Copy Number Variant and the RBFOX1 Gene
- Brain organoids: A revolutionary tool for modeling neurological disorders and development of therapeutics
- Single-cell brain organoid screening identifies developmental defects in autism
- Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells
- Application of organoids in regenerative medicine
- Human iPSC-derived retinal organoids develop robust Alzheimer's disease neuropathology
- Schizophrenia endothelial cells exhibit higher permeability and altered angiogenesis patterns in patient-derived organoids
- Cell-type specific defects in PTEN-mutant cortical organoids converge on abnormal circuit activity
- Optimization of differential filtration-based mitochondrial isolation for mitochondrial transplant to cerebral organoids
- ASCL1 induces neurogenesis in human Müller glia
- Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light
- Mass Spectrometry Reveals that Oxysterols are Secreted from Non-Alcoholic Fatty Liver Disease Induced Organoids
- Effects of acute low-moderate dose ionizing radiation to human brain organoids
- Single-cell long-read sequencing in human cerebral organoids uncovers cell-type-specific and autism-associated exons
- Patient- and xenograft-derived organoids recapitulate pediatric brain tumor features and patient treatments
- Germline PTEN genotype-dependent phenotypic divergence during the early neural developmental process of forebrain organoids
- Advances in the knowledge and therapeutics of schizophrenia, major depression disorder, and bipolar disorder from human brain organoid research
- APOE3 Christchurch modulates β-catenin/Wnt signaling in iPS cell-derived cerebral organoids from Alzheimer's cases
- A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
- Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases
- Altered neuroepithelial morphogenesis and migration defects in iPSC-derived cerebral organoids and 2D neural stem cells in familial bipolar disorder
- Human pluripotent stem cell (hPSC) and organoid models of autism: opportunities and limitations
- Induced Pluripotent Stem Cells and Organoids in Advancing Neuropathology Research and Therapies
- Cross-species analysis identifies mitochondrial dysregulation as a functional consequence of the schizophrenia-associated 3q29 deletion
- Morphological and electrophysiological characterization of a novel displaced astrocyte in the mouse retina
- Evaluation of the efficacy of mitochondrial fission inhibitor (Mdivi-1) using non-alcoholic steatohepatitis (NASH) liver organoids
- Generation of human cerebral organoids with a structured outer subventricular zone
- Schizophrenia-associated NRXN1 deletions induce developmental-timing- and cell-type-specific vulnerabilities in human brain organoids
- Schizophrenia-associated NRXN1 deletions induce developmental-timing- and cell-type-specific vulnerabilities in human brain organoids
- Profiling transcriptomic responses of human stem cell-derived medium spiny neuron-like cells to exogenous phasic and tonic neurotransmitters
- The NAD+ Precursor Nicotinamide Riboside Rescues Mitochondrial Defects and Neuronal Loss in iPSC derived Cortical Organoid of Alpers' Disease
- Generation and characterization of mature hepatocyte organoids for liver metabolic studies
- Kirigami electronics for long-term electrophysiological recording of human neural organoids and assembloids
- The temporal progression of retinal degeneration and early-stage idebenone treatment in the Pde6brd1/rd1 mouse model of retinal dystrophy
- iPSC-derived models for anorexia nervosa research
- Stem cell modeling of nervous system tumors
- ARID1B controls transcriptional programs of axon projection in an organoid model of the human corpus callosum
- Biological agents and the aging brain: glial inflammation and neurotoxic signaling
- Vascularized Brain Assembloids with Enhanced Cellular Complexity Provide Insights into The Cellular Deficits of Tauopathy
- LIF signaling regulates outer radial glial to interneuron fate during human cortical development
- A common single nucleotide variant in the cytokine receptor-like factor-3 (CRLF3) gene causes neuronal deficits in human and mouse cells
- Extracellular vesicles secreted by 3D tumor organoids are enriched for immune regulatory signaling biomolecules compared to conventional 2D glioblastoma cell systems
- Three-dimensional ultrastructure of the normal rod photoreceptor synapse and degenerative changes induced by retinal detachment
- Induced pluripotent stem cell models as a tool to investigate and test fluid biomarkers in Alzheimer's disease and frontotemporal dementia
- Differential expression analysis identifies candidate synaptogenic molecules for wiring direction-selective circuits in the retina
- Modeling idiopathic autism in forebrain organoids reveals an imbalance of excitatory cortical neuron subtypes during early neurogenesis
- The Bovine Ex Vivo Retina: A Versatile Model for Retinal Neuroscience
- PSEN1 E280A Cholinergic-like Neurons and Cerebral Spheroids Derived from Mesenchymal Stromal Cells and from Induced Pluripotent Stem Cells Are Neuropathologically Equivalent
- Physical activity and the brain myelin content in humans
- Characterization of enhancer activity in early human neurodevelopment using Massively Parallel Reporter Assay (MPRA) and forebrain organoids
- TBC1D32 variants disrupt retinal ciliogenesis and cause retinitis pigmentosa
- Cell-specific transcriptome changes in the hypothalamic arcuate nucleus in a mouse deoxycorticosterone acetate-salt model of hypertension
- Customized liver organoids as an advanced in vitro modeling and drug discovery platform for non-alcoholic fatty liver diseases
- Identifying Hmga2 preserving visual function by promoting a shift of Müller glia cell fate in mice with acute retinal injury
- Identifying Hmga2 preserving visual function by promoting a shift of Müller glia cell fate in mice with acute retinal injury
- Neurodevelopmental defects in human cortical organoids with N-acetylneuraminic acid synthase mutation
- Neuronal dysfunction and gene modulation by non-coding RNA in Parkinson's disease and synucleinopathies
- Recapitulation of Perturbed Striatal Gene Expression Dynamics of Donor's Brains With Ventral Forebrain Organoids Derived From the Same Individuals With Schizophrenia
- The light-dependent pseudo-capacitive charging of conjugated polymer nanoparticles coupled with the depolarization of the neuronal membrane
- Osteopontin drives neuroinflammation and cell loss in MAPT-N279K frontotemporal dementia patient neurons
- Understanding the neurological implications of acute and long COVID using brain organoids
- Distinct ultrastructural phenotypes of glial and neuronal alpha-synuclein inclusions in multiple system atrophy
- Biomarkers associated with the pathogenesis of Alzheimer's disease