Evidence
Nanoscale. 2023 May 9. doi: 10.1039/d3nr00305a. Online ahead of print.
ABSTRACT
As a super-resolution imaging method, stimulated emission depletion (STED) microscopy has unraveled fine intracellular structures and provided insights into nanoscale organizations in cells. Although image resolution can be further enhanced by continuously increasing the STED-beam power, the resulting photodamage and phototoxicity are major issues for real-world applications of STED microscopy. Here we demonstrate that, with 50% less STED-beam power, the STED image resolution can be improved up to 1.45-fold using the separation of photons by a lifetime tuning (SPLIT) scheme combined with a deep learning-based phasor analysis algorithm termed flimGANE (fluorescence lifetime imaging based on a generative adversarial network). This work offers a new approach for STED imaging in situations where only a limited photon budget is available.
PMID:37159237 | DOI:10.1039/d3nr00305a
Add to Google Keep
Estimated reading time: 3 minute(s)
Latest: Psychiatryai.com #RAISR4D
Real-Time Evidence Search [Psychiatry]
Spatial resolution enhancement in photon-starved STED imaging using deep learning-based fluorescence lifetime analysis
🌐 90 Days
VR Related Evidence Matrix
- Super-resolution neural networks improve the spatiotemporal resolution of adaptive MRI-guided radiation therapy
- Altered TFEB subcellular localization in nigral neurons of subjects with incidental, sporadic and GBA-related Lewy body diseases
- Atomic Force Microscopy beyond Topography: Chemical Sensing of 2D Material Surfaces through Adhesion Measurements
- Impact of PET Reconstruction on Amyloid-β Quantitation in Cross-Sectional and Longitudinal Analyses
- A polarized cell system amenable to subcellular resolution imaging of influenza virus infection
- Advanced feature learning and classification of microscopic breast abnormalities using a robust deep transfer learning technique
- Advancements in high-resolution 3D microscopy analysis of endosomal morphology in postmortem Alzheimer's disease brains
- Tripeptidyl peptidase II coordinates the homeostasis of calcium and lipids in the central nervous system and its depletion causes presenile dementia in female mice through calcium/lipid dyshomeostasis-induced autophagic degradation of CYP19A1
- Concurrent functional ultrasound imaging with graphene-based DC-coupled electrophysiology as a platform to study slow brain signals and cerebral blood flow under control and pathophysiological brain states
- AI-assisted mass spectrometry imaging with in situ image segmentation for subcellular metabolomics analysis
- Optical Estimation of Membrane Potential Values Using Fluorescence Lifetime Imaging Microscopy and Hybrid Chemical-Genetic Voltage Indicators
- Use and performance of artificial intelligence applications in the diagnosis of chronic apical periodontitis based on cone beam computed tomography imaging
- Proteomics Impact on Cell Biology to Resolve Cell Structure and Function
- Brain-machine interface based on deep learning to control asynchronously a lower-limb robotic exoskeleton: a case-of-study
- US2Mask: Image-to-mask generation learning via a conditional GAN for cardiac ultrasound image segmentation
- A Comparative Analysis of Deep Learning-Based Approaches for Classifying Dental Implants Decision Support System
- Based on neural network cascade abnormal texture information dissemination of classification of patients with schizophrenia and depression
- Artificial Intelligence in Nuclear Cardiology: An Update and Future Trends
- DeepPI: Alignment-Free Analysis of Flexible Length Proteins Based on Deep Learning and Image Generator
- Discontinuity third harmonic generation microscopy for label-free imaging and quantification of intraepidermal nerve fibers
- The UK National Recovery Survey: nationally representative survey of people overcoming a drug or alcohol problem
- A supervised, externally validated machine learning model for artifact and drainage detection in high-resolution intracranial pressure monitoring data
- Intraoperative Videogrammetry and Photogrammetry for Photorealistic Neurosurgical 3-Dimensional Models Generated Using Operative Microscope: Technical Note
- Unsupervised deep representation learning enables phenotype discovery for genetic association studies of brain imaging
- Diagnostic power of ChatGPT 4 in distal radius fracture detection through wrist radiographs
- Deep learning applications in vascular dementia using neuroimaging
- Revealing speckle obscured living human retinal cells with artificial intelligence assisted adaptive optics optical coherence tomography
- ThyroidNet: A Deep Learning Network for Localization and Classification of Thyroid Nodules
- AI-based support for optical coherence tomography in age-related macular degeneration
- Toolkits for detailed and high-throughput interrogation of synapses in C. elegans
- MnO2/Ce6 microbubble-mediated hypoxia modulation for enhancing sono-photodynamic therapy against triple negative breast cancer
- Towards biologically plausible phosphene simulation for the differentiable optimization of visual cortical prostheses
- An actor-model framework for visual sensory encoding
- Impact of Deep Learning Image Reconstruction Methods on MRI Throughput
- Multiple Classification of Brain MRI Autism Spectrum Disorder by Age and Gender Using Deep Learning
- Neuropathological Applications of Microscopy with Ultraviolet Surface Excitation (MUSE): A Concordance Study of Human Primary and Metastatic Brain Tumors
- Deep Learning-Based Automated Detection of Retinal Breaks and Detachments on Fundus Photography
- A medical image segmentation method for rectal tumors based on multi-scale feature retention and multiple attention mechanisms
- Application of deep learning in radiation therapy for cancer
- High-resolution EEG source localization in personalized segmentation-free head model with multi-dipole fitting
- A deep learning approach for Direct Immunofluorescence pattern recognition of Autoimmune Bullous Diseases
- Crop filling: A pipeline for repairing memory clinic MRI corrupted by partial brain coverage
- Care giving and receiving for people with complex emotional needs within a crisis resolution/home treatment setting: A qualitative evidence synthesis
- Tracking subjects' strategies in behavioural choice experiments at trial resolution
- Integrating portable NIR spectrometry with deep learning for accurate Estimation of crude protein in corn feed
- Protein-Based Tools for Studying Neuromodulation
- Macaque Brainnetome Atlas: A multifaceted brain map with parcellation, connection, and histology
- Structure of alpha-synuclein fibrils derived from human Lewy body dementia tissue
- DC2Net: An Asian Soybean Rust Detection Model Based on Hyperspectral Imaging and Deep Learning
- Real-time single-molecule imaging of CaMKII-calmodulin interactions
- Mobile applications in COVID-19 detection and diagnosis: an efficient tool to control the future pandemic; a multidimensional systematic review of the state of the art
- Performance of non-invasive tests for liver fibrosis and resolution after bariatric surgery
- Conditional Diffusion Models for Semantic 3D Brain MRI Synthesis
- A consistent map in the medial entorhinal cortex supports spatial memory
- Some new insights into the psychology of individuals and large groups in a world of changing conflicts
- The new era of artificial intelligence in neuroradiology: current research and promising tools
- High-speed measurements of SNARE-complexin interactions using magnetic tweezers
- Simulation-based education in ultrasound - diagnostic and interventional abdominal focus
- Mapping dynamic spatial patterns of brain function with spatial-wise attention
- Iterative reconstruction for limited-angle CT using implicit neural representation
- Reproducibility of arterial spin labeling cerebral blood flow image processing: A report of the ISMRM open science initiative for perfusion imaging (OSIPI)_and the ASL MRI challenge
- Endothelial receptor proteins in acute venous thrombosis and delayed thrombus resolution in cerebral sinus vein thrombosis
- Raman-AFM-fluorescence-guided impact of linoleic and eicosapentaenoic acids on subcellular structure and chemical composition of normal and cancer human colon cells
- Stochastic Optical Reconstruction Microscopy Imaging of Multiple System Atrophy Inclusions Suggests Stepwise α-Synuclein Aggregation
Evidence Blueprint
Spatial resolution enhancement in photon-starved STED imaging using deep learning-based fluorescence lifetime analysis
☊ AI-Driven Related Evidence Nodes
(recent articles with at least 5 words in title)
Save Evidence Blueprint
Save as PDF
Spatial resolution enhancement in photon-starved STED imaging using deep learning-based fluorescence lifetime analysis
🌐 365 Days
VR Related Evidence Matrix
- The interwoven fibril-like structure of amyloid-beta plaques in mouse brain tissue visualized using super-resolution STED microscopy
- Super-resolution neural networks improve the spatiotemporal resolution of adaptive MRI-guided radiation therapy
- The impact of chemical fixation on the microanatomy of mouse organotypic hippocampal slices
- Resolution enhancement with a task-assisted GAN to guide optical nanoscopy image analysis and acquisition
- Quantifying Intracellular Nanoparticle Distributions with Three-Dimensional Super-Resolution Microscopy
- Comparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain
- Imaging brain tissue architecture across millimeter to nanometer scales
- Adaptive Optical Two-Photon Fluorescence Microscopy Probes Cellular Organization of Ocular Lenses In Vivo
- 'Cryo Super-Resolution Fluorescence Microscopy for Correlation with Cryo-ET’
- Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology
- Tissue-Like 3D Standard and Protocols for Microscope Quality Management
- Innovative advances in pediatric radiology: computed tomography reconstruction techniques, photon-counting detector computed tomography, and beyond
- Altered TFEB subcellular localization in nigral neurons of subjects with incidental, sporadic and GBA-related Lewy body diseases
- Deciphering tumor ecosystems at super resolution from spatial transcriptomics with TESLA
- Diffuse Optical Tomography Spatial Prior for EEG Source Localization in Human Visual Cortex
- Application of image super-resolution recognition and artificial intelligence system in repairing students' psychological education problems
- Atomic Force Microscopy beyond Topography: Chemical Sensing of 2D Material Surfaces through Adhesion Measurements
- Recent advances in in-resin correlative light and electron microscopy of Epon-embedded cells
- DEEP-squared: deep learning powered De-scattering with Excitation Patterning
- Neurons on tape: Automated Tape Collecting Ultramicrotomy-mediated volume EM for targeting neuropathology
- SF2Former: Amyotrophic lateral sclerosis identification from multi-center MRI data using spatial and frequency fusion transformer
- Impact of PET Reconstruction on Amyloid-β Quantitation in Cross-Sectional and Longitudinal Analyses
- A polarized cell system amenable to subcellular resolution imaging of influenza virus infection
- Advanced feature learning and classification of microscopic breast abnormalities using a robust deep transfer learning technique
- High-resolution imaging in studies of alcohol effect on prenatal development
- A review on deep learning applications in highly multiplexed tissue imaging data analysis
- MiniMounter: a low-cost miniaturized microscopy development toolkit for image quality control and enhancement
- The Effect of Diabetes Mellitus Type 1 on the Energy Metabolism of Hepatocytes: Multiphoton Microscopy and Fluorescence Lifetime Imaging
- Advancements in high-resolution 3D microscopy analysis of endosomal morphology in postmortem Alzheimer's disease brains
- Upconversion-Luminescent Fiber Microchannel Sensors for Temperature Monitoring at High Spatial Resolution in the Brains of Freely Moving Animals
- Tripeptidyl peptidase II coordinates the homeostasis of calcium and lipids in the central nervous system and its depletion causes presenile dementia in female mice through calcium/lipid dyshomeostasis-induced autophagic degradation of CYP19A1
- Concurrent functional ultrasound imaging with graphene-based DC-coupled electrophysiology as a platform to study slow brain signals and cerebral blood flow under control and pathophysiological brain states
- Deep learning based phenotyping of medical images improves power for gene discovery of complex disease
- Multimodal anatomical mapping of subcortical regions in Marmoset monkeys using high-resolution MRI and matched histology with multiple stains
- Quantitative assessment of hepatic steatosis using label-free multiphoton imaging and customized image processing program
- AI-assisted mass spectrometry imaging with in situ image segmentation for subcellular metabolomics analysis
- Serial Block Face-Scanning Electron Microscopy as a Burgeoning Technology
- Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabeling Electron Microscopy
- Optical Estimation of Membrane Potential Values Using Fluorescence Lifetime Imaging Microscopy and Hybrid Chemical-Genetic Voltage Indicators
- Correlative light and electron microscopy to explore the lytic immunological synapse between natural killer cells and cancer cells
- Location, location, location: Protein kinase nanoclustering for optimised signalling output
- Spectrofluorometric quantitative analysis of aripiprazole based on quenching of natural derived carbon quantum dots in spiked human plasma
- Detection of atmospheric aerosols and terrestrial nanoparticles collected in a populous city in southern Brazil
- Three-dimensional visualization and analysis of dendritic spines in human brain tissue
- Use and performance of artificial intelligence applications in the diagnosis of chronic apical periodontitis based on cone beam computed tomography imaging
- PS-Net: Human perception-guided segmentation network for EM cell membrane
- MULTI-TASK DEEP LEARNING AND UNCERTAINTY ESTIMATION FOR PET HEAD MOTION CORRECTION
- Proteomics Impact on Cell Biology to Resolve Cell Structure and Function
- Deep Learning and Geriatric Mental Health
- Autism spectrum disorder diagnosis based on deep unrolling-based spatial constraint representation
- Deep Learning Network with Spatial Attention Module for Detecting Acute Bilirubin Encephalopathy in Newborns Based on Multimodal MRI
- Deep Learning Network with Spatial Attention Module for Detecting Acute Bilirubin Encephalopathy in Newborns Based on Multimodal MRI
- High-resolution structural and functional retinal imaging in the awake behaving mouse
- Brain-machine interface based on deep learning to control asynchronously a lower-limb robotic exoskeleton: a case-of-study
- Segmented spin-echo echo-planar imaging improves whole-brain BOLD functional MRI in awake pigeon brains
- US2Mask: Image-to-mask generation learning via a conditional GAN for cardiac ultrasound image segmentation
- Brain age prediction using combined deep convolutional neural network and multi-layer perceptron algorithms
- Guava-fruit based synthesis of carbon quantum dots for spectrofluorometric quantitative analysis of risperidone in spiked human plasma and pharmaceutical dosage forms
- A Deep Learning Approach for Neuronal Cell Body Segmentation in Neurons Expressing GCaMP using a Swin Transformer
- Deep-brain optical recording of neural dynamics during behavior
- A Comparative Analysis of Deep Learning-Based Approaches for Classifying Dental Implants Decision Support System
- Based on neural network cascade abnormal texture information dissemination of classification of patients with schizophrenia and depression
- Artificial Intelligence in Nuclear Cardiology: An Update and Future Trends
- Mapping high-grade glioma immune infiltration to 5-ALA fluorescence levels: TCGA data computation, classical histology, and digital image analysis