- Amylin-immunoreactive cells located in pancreatic islets; antibody specificity confirmed by loss of staining after preabsorption with synthetic amylin.
- Nearly all amylin-immunoreactive cells co-express peptide YY, indicating likely co-secretion and intrainsular modulation of insulin and glucagon secretion.
- A subpopulation of weakly glucagon- and GLP-1-immunoreactive cells corresponds to amylin/PYY cells, suggesting co-secretion that may also modulate food intake centrally.
Anat Histol Embryol. 2026 Sep;55(5):e70161. doi: 10.1111/ahe.70161.
ABSTRACT
The distribution of amylin-like substances was investigated by immunohistochemistry in the pancreas of the Japanese eel (Anguilla japonica). Amylin-immunoreactive cells were observed in pancreatic islets. These immunostaining profiles were not observed by the preabsorption of the anti-amylin serum with synthetic amylin, indicating that the serum specifically recognized eel amylin-like peptide. Almost all amylin-immunoreactive cells were immunopositive for peptide YY, suggesting that amylin and peptide YY co-secreted from the islet cells might participate in the modulation of insulin and glucagon secretion in the pancreatic islets. In addition, weakly glucagon-immunopositive cells, but not densely glucagon-immunopositive cells, also corresponded to amylin-immunoreactive cells. The subpopulation of glucagon-immunoreactive and glucagon-like peptide-1-immunoreactive cells was also immunopositive for peptide YY. These results suggest that amylin and peptide YY, and also presumably glucagon-like peptide-1, are co-secreted from the amylin/peptide YY cells, and these three peptides modulate food intake through the central nervous system in addition to intrainsular functions.
PMID:42559671 | DOI:10.1111/ahe.70161
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