- Comprehensive phytochemical profiling identified 57 GC-MS compounds (73.65% fatty acids and esters) and major UHPLC-MS/MS constituents including 2',4'-dihydroxychalcone (20.72%).
- Hydro-alcoholic extract exhibited potent antioxidant activity (ABTS IC50 7.57 µg/mL) and high SPF (40.56); chloroform fraction inhibited α-amylase and BChE.
- Molecular docking indicated a stable but antagonistic 3-tetradecyn-1-ol and α-amylase complex (−4.8 kcal/mol); authors call for further in vivo validation.
Chem Biodivers. 2026 Aug;23(8):e71578. doi: 10.1002/cbdv.71578.
ABSTRACT
Natural remedies combined with bioinformatics accelerate the formulation of safer, targeted drugs. This study provides a comprehensive description of the phytochemical and pharmacological aspects of Silene colorata. Binding profiles of eight identified compounds were simulated against α-amylase. GC-MS analysis revealed 57 compounds, mainly fatty acids and esters (73.65%), while UHPLC-MS/MS identified 2′,4′-dihydroxychalcone (20.72%), (epi)gallocatechin-C-hexoside (12.53%), apigetrin (10.10%), and hexahydroxyheptanoic acid (8.57%) as major components. The hydro-alcoholic extract showed a strong antiradical effect (IC50 = 7.57 ± 0.26 µg/mL by ABTS assay; A0.50 = 48.93 ± 3.88 µg/mL by phenanthroline assay). Its sun protection factor (SPF) was also the highest (40.561 ± 0.87). The chloroform fraction showed notable activity against α-amylase (IC50 = 22.86 ± 3.19 µg/mL) and butyrylcholinesterase (IC50 = 111.08 ± 4.72 µg/mL). The complex 3-tetradecyn-1-ol and α-amylase appeared more stable, but in an antagonistic manner (-4.8 kcal/mol). While this work reveals crucial findings, further in vivo studies are needed to confirm and expand on these results.
PMID:42555847 | DOI:10.1002/cbdv.71578
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